As a postdoctoral researcher Darren developed a self-biotinylating lentiviral 293T HEK packaging cell line with Professors Farzin Farzaneh (Kings College London) and Nigel Slater (Cambs). He also constructed single chain trimer versions of the human MHC Class I complex with Dr Keith Gould at Imperial College to investigate the aetiology of spondyloarthritis. Darren's postdoctoral research at UCL with Professors Eli Keshavarz-Moore and John Ward involved engineering Escherichia coli cells for improved bioprocessing of antibody-based therapeutics. Darren completed his PhD with Professor Mark Field working on intracellular trafficking in Chinese hamster ovary (CHO) cells and the protozoan Trypanosoma brucei. After his PhD he took a 2-year career break from research to work in the City of London advising biotechnology and Internet companies on financial communication strategy as they sought initial public offering (IPO) on UK and European equity markets.